G6PD deficiency is an inherited condition in which the body is deficient in the enzyme glucose-6-phosphate dehydrogenase, or G6PD, which helps red blood cells (RBCs) function normally. This deficiency can cause hemolytic anemia, usually after exposure to certain medications, foods, or even infections. Early detection of this enzyme deficiency can aid in the diagnosis and treatment.
Glucose-6-phosphate dehydrogenase is the initial enzyme in the hexose monophosphate pathway of glucose metabolism. In the SPOTCHECK® method, enzyme activity is measured by observing the fluorescence produced after NADP+ is reduced to NADPH when glucose-6-phosphate is present as a substrate. As G6PD catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconate, NADP+ is reduced to NADPH, which is measured by a fluorometer. The amount of NADPH produced is proportional to the G6PD enzyme activity in the sample. Maleimide, an inhibitor of 6-phosphogluconate dehydrogenase activity, is added to prevent the conversion of 6-phosphogluconate to ribulose-5-phosphate and additional production of NADPH.
G6PD is analyzed on the SPOTCHECK Flow System
Positive Attributes of Instrumentation and Method:
- Semi-quantitative results
- No operator attendance is required after loading sample plate
- Modular system for easy maintenance
- Large capacity autosampler
- Same day turnaround of results
- Totally automatic – no transfer of sample or reagents from one microtiter plate to another.
- Throughput is about 90 samples per hour.
- Can be run simultaneously with Total Galactose and Phenylalanine assays